LOCUS       62056_481       10850 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10850)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6715..6756
                     /codon_start=1
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
                     /translation="GKPIPNPLLGLDST"
     CDS             6766..6783
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     polyA_signal    6812..7036
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7082..7510
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7524..7853
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        7901..7948
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             7967..8362
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8523..8656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(8741..8771)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8786..8807)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9095..9683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9857..10714)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10715..10819)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 atgcagcatc aacggcagca gcaagaggac cacgcgaacg aggcaaacta tgcaagaggc
      961 accagactcc ctatttctgg tgaaggacca acttctcagc cgaacagctc caagcaaacc
     1021 gtcctgtctt ggcaagctgc aattgatgct gctagacagg ccaaggccgc ccagaccatg
     1081 agcacgtctg cacccccacc tgtaggatct ctctcccaaa gaaaacgtca gcaatacgcc
     1141 aagagcaaaa aacagggtaa ctcgtctaac agccgacctg cccgtgccct cttctgttta
     1201 tccctcaata accccatccg aagagcctgc attagtatag tggattggaa accatttgac
     1261 atatttatat tattggctat ttttgccaat tgtgtggcct tagctattta catcccgttc
     1321 cctgaagatg attctaattc aacaaatcat aacttggaaa aagtagaata tgccttcctg
     1381 attatcttta cagtggagac atttctgaag attatagcgt acggactgct gctgcatcct
     1441 aatgcttacg ttaggaacgg atggaactta ctggattttg ttatagtaat agtaggattg
     1501 tttagtgtaa ttttggaaca attaaccaaa gaaacagaag gcgggaacca ctctagtggc
     1561 aaatcaggag gctttgatgt caaagccctc cgtgccttcc gagtgttaag accgcttcga
     1621 cttgtatcat cagtgcccag tttacaagtt gttctgaact ccattataaa agccatggtc
     1681 cccctcctcc acatagccct tttggtgtta ttcgtaatta tcatctatgc gattataggg
     1741 ttggaacttt ttattggaaa aatgcacaaa acctgttttt ttgctgactc agatatagta
     1801 gctgaagagg acccagctcc atgcgcgttc tcagggaatg gacgccagtg tgccgccaat
     1861 ggcacggaat gtaggagcgg ctgggtcggc cccaatggag gcatcactaa ttttgacaac
     1921 tttgcctttg ccatgctcac tgtgttccag tgcatcacca tggagggctg gacagatgtg
     1981 ctctactggg ttaatgatgc gataggatgg gagtggccat gggtgtattt tgttagtctg
     2041 atcatccttg gctcattttt cgtccttaac ctggttcttg gtgtccttag tggagaattc
     2101 tcaaaggaaa gagagaaggc taaagcacgt ggagatttcc agaagcttcg ggagaagcag
     2161 cagctggaag aggatctcaa gggctacttg gactggatca cccaggcaga agacatagat
     2221 cctgagaatg aagaggaggg tggagaggag ggcaaacgaa acactagcat gcccaccagt
     2281 gagaccgaat ctgtgaacac agagaacgta agtggtgaag gcgagaccca gggatgctgt
     2341 ggaagtctct ggtgctggtg gaaaagaaga ggcgcggcca agactgggcc ctctgggtgt
     2401 cggcggtggg gccaagccat ctcgaaatcc aagctcagcc gtcggtggcg tcgctggaac
     2461 cggttcaatc gcaggagatg cagggcagct gtgaagtctg tcacgtttta ctggctggtg
     2521 atcgttctgg tgtttctcaa cactctgacg atttcctctg agcactacaa tcagccagac
     2581 tggctgacgc agattcaaga tatagcgaac aaagtgctct tggctttgtt cacctgtgag
     2641 atgctggtga agatgtacag cctgggcctc caggcctact tcgtctctct cttcaaccgc
     2701 ttcgattgct ttgtggtgtg tgggggaatc accgaaacca tcctggtgga actggagctc
     2761 atgtctcccc tgggcgtctc tgtgttccgg tgtgtacgtc tcctgaggat cttcaaagtg
     2821 accaggcact ggacttccct gagcaacttg gtggcgtccc tgttaaactc catgaagtcc
     2881 atcgcctctc tgctgttgct gctcttcctc ttcatcatca tcttctccct gctggggatg
     2941 cagctgttcg gagggaagtt caatttcgat gagacacaga ccaagcgaag caccttcgac
     3001 aacttcccac aggcgctcct gactgtgttc cagatcctga cgggcgaaga ctggaatgcg
     3061 gtgatgtatg acggcatcat ggcctacgga ggcccgtcct cctctggaat gattgtctgt
     3121 atttacttca tcatcctttt catctgtggt aactatatcc tgctgaacgt cttcttggcc
     3181 attgctgtag acaatttggc tgatgctgaa agtctgaaca ctgctcagaa agaggaagcc
     3241 gaagaaaaag aaaggaaaaa aattgccaga aaagaaagcc tagaaaacaa aaaaaacaac
     3301 aaaccagagg tcaaccagat agccaacagt gacaacaagg ttactattga tgactatcaa
     3361 gaggaggctg aagataagga cccttatcca ccctgcgatg tgccagtagg tgaagaggaa
     3421 gaggaggagg aagaggacga gcctgaggtt cctgctggtc cccgtcctcg aagaatctca
     3481 gagttgaaca tgaaggagaa aattgcgccc atccccgaag gaagcgcatt cttcattctt
     3541 agcaagacca acccgatccg tgtgggttgc cacaaactca tcaaccacca catcttcacc
     3601 aacctcatcc tggtcttcat catgctgagc agcgctgccc tggccgcaga ggatcccatc
     3661 cgcagccact ccttccgcaa cactatactg ggctactttg actatgcttt cacagccatc
     3721 tttacggttg aaatcctgtt aaagatgaca acttttggag cgttcttgca caaaggggct
     3781 ttctgtagga actacttcaa tttgctggac atgctggtcg ttggggtgtc tctggtgtca
     3841 tttgggattc aatccagtgc catctcggtt gtgaagattc tgagggtctt aagggtcttg
     3901 aggcctctca gagcaatcaa cagagcaaaa ggacttaagc acgtggtcca gtgtgtcttt
     3961 gtggccatcc gaaccatcgg caacatcatg atcgtcacga ccctgctcca gttcatgttt
     4021 gcttgcattg gggtccagct gttcaagggg aagttctacc gttgcacaga tgaggccaaa
     4081 agtaaccccg aggagtgcag ggggcttttc atcctttata aggacggcga tgtcgacagt
     4141 cccgtggtcc gtgagaggat ctggcaaaac agtgatttca atttcgacaa tgtcctttcg
     4201 gctatgatgg tgctcttcac ggtctcgact tttgagggct ggcccgcgtt gctgtacaaa
     4261 gctattgact caaacggaga gaacgttggt cctgtctaca actaccgtgt ggagatctcc
     4321 atcttcttca ttatctacat catcatcgtg gccttcttca tgatgaatat cttcgtgggc
     4381 ttcgtcatcg tcaccttcca ggagcaggga gaaaaggagt ataagaactg tgagctggac
     4441 aaaaatcagc gtcagtgtgt ggaatatgcc ttgaaggccc gccccttaag gagatacatc
     4501 cccaaaaacc cataccagta caagttctgg tacgtggtga actcctcgcc tttcgaatat
     4561 atgatgtttg tcctcatcat gctcaacacg ctctgcctgg ccatgcagca ctatgagcaa
     4621 tccaagatgt tcaatgacgc catggacatt ctgaacatgg tcttcacggg ggtcttcacc
     4681 gttgagatgg ttttgaaagt catcgcattt aagcccaagc actatttcac tgatgcatgg
     4741 aatacttttg atgccttaat tgttgtcggt agcgtcgttg atattgctat aactgaagtg
     4801 aataactctg aagagagcaa tagaatctcc atcacctttt tccgtctttt ccgagtgatg
     4861 cggttggtga agcttctcag cagaggggaa ggcatccgga ctctgctatg gaccttcatt
     4921 aagtccttcc aggcactccc atatgtcgcc ctcctcattg ccatgctctt cttcatctac
     4981 gccgtcattg gcatgcagat gtttgggaag gttgccatga gagataacaa ccagatcaat
     5041 aggaacaaca acttccagac gtttccccag gcagtgctgc tgctcttcag gtgtgcaaca
     5101 ggggaggcct ggcaggagat catgctcgcc tgcctccctg ggaagctgtg tgacccggac
     5161 tcagattaca acccaggaga ggaatatact tgtgggagca actttgccat tgtctacttc
     5221 atcagctttt acatgctctg tgcgttcctg atcatcaacc tcttcgtggc tgtcatcatg
     5281 gacaattttg actatctgac gagggactgg tctattctgg ggcctcacca cttggacgaa
     5341 ttcaaaagaa tatggtctga atatgacccc gaggcaaagg gaaggataaa acaccttgat
     5401 gtggtcactt tgctccgacg tatccagcct cccctgggat ttggaaagtt gtgtccacac
     5461 cgagtggcgt gtaagagatt ggtcgccatg aacatgcctc tcaacagtga tgggacagtc
     5521 atgttcaatg caactctgtt tgctttggta cggacggctc tcaagatcaa gactgaaggc
     5581 aacctggagc aagctaatga agagctccgc gctgtgataa agaaaatctg gaagaagaca
     5641 agcatgaagc tacttgacca agttgtccct ccagctggtg atgatgaggt aaccgtgggg
     5701 aagttctatg ctactttcct gatacaggac tactttagga aattcaagaa gcggaaagag
     5761 caaggcctgg tggggaaata ccctgcgaag aacaccacga tcgccctaca gatgcttgaa
     5821 cggatgctct agagttgtct gcctgagcta ccgcaccaag ctggttagtc cggaggcgct
     5881 gtggctaagg ccttgaaagg gcaaactccc cctcgagctt atttctactg aagcagcatt
     5941 tcaggagaaa tgcaggcacc aaagccctgg ggagacccct tgcgagcaag gcactcctcc
     6001 ggaaggaagg aaggaaggca gaagccgggc tgtgtgtggc tcatctgtgg agccctggct
     6061 gcctgttgcg gacactgctc atggtggctc cttttcatga aagggaacat atgagttatg
     6121 ttttcccctt ccaagccaga gactcagtga gagggaaaga aagagagaga gggagggaga
     6181 gagggagaga gagaacgcaa aggggcagcc accccacaga gaaatcaaat gaacacaact
     6241 tgctgacgat ggccagtgaa taaaataaat aatgttttta atatatatat atatacatat
     6301 atatgtgtat gtatatatat atgactaagc agacctgtgt aaggcagaca acggactctg
     6361 ccccaaagtc ctgcatccct agtttgcccg gactcccaaa gactggcagg ctgattggaa
     6421 ctgagcagac agagttcctt tcctgtgcca gcacgtcaca acctcctgac cacccggccc
     6481 tgcttgctgc agtgacctag tccagaatgc actgtaggtg gaaggccagg aagctcagac
     6541 acacccattg aaactaacac atacacccgc atgccaaaac caaccaggca acagctcagg
     6601 ttccgtctcc cctaccgtgt ccgcggggaa acgccctcgt gccgaattcc tgcagcccgg
     6661 gggatccact agttctagag cggccgctcg agtctagagg gcccgcggtt cgaaggtaag
     6721 cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac
     6781 cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     6841 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     6901 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     6961 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     7021 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     7081 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7141 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7201 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7261 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7321 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7381 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7441 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7501 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     7561 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     7621 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7681 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     7741 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     7801 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     7861 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     7921 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt
     7981 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
     8041 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
     8101 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
     8161 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
     8221 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
     8281 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
     8341 ctctggttat gtgtgggagg gctaagcact tcgtggccga ggagcaggac tgacacgtgc
     8401 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     8461 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     8521 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8581 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8641 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     8701 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8761 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8821 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8881 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8941 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9001 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9061 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     9541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    10021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    10081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    10141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    10201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
